Biotic Interactions across Gradients and Scales provides a valuable exploration of the intricate connections of the web of life, including foundational ecological principles to current macroecological perspectives. The book examines the various dimensions of biotic interactions across spatiotemporal gradients in terrestrial and aquatic environments. Its integrated approach is crucial to understanding the potential responses of ecological networks to unprecedented stressors, including climate change, anthropogenic impacts, and loss of connectivity and functional redundancy. Written by renowned subject matter experts from the global North and South, this book introduces the concept of biotic interactions before delving into the physical factors influencing ecological networks. Central chapters describe the tools, methods, and history of study of biotic interactions. Latter chapters explore such networks across aquatic and terrestrial domains, before concluding chapters discuss disruptive stressors and open science opportunities for future research endeavors. Case studies, boxed elements, and end chapter review questions ensure student mastery of content.