Studies on gender and sexuality have proliferated in the last decades, covering a wide spectrum of disciplines. This collection of essays offers a metanarrative of sexuality as it has been recently embedded in the art historical discourse of the European Renaissance. It revisits ‘canonical’ forms of visual culture, such as painting, sculpture and a number of emblematic manuscripts. The contributors focus on one image—either actual or thematic—and examine it against its historiographic assumptions. Through the use of interdisciplinary approaches, the essays propose to unmask the ideology(ies) of representation of sexuality and suggest a richer image of the ever-shifting identities of gender. The collection focuses on the Italian Renaissance, but also includes case studies from Germany and France.