Alternatives to Antibiotics in Sustainable Aquaculture explores issues associated with antimicrobial use and available alternatives to support aquatic animal health. The book unveils the issues related to antimicrobial use and offers alternative approaches to foster a more sustainable global aquaculture industry. These approaches encompass natural-based antibiotic substitutes, antimicrobial peptides, probiotics, prebiotics, and postbiotics, as well as immunostimulants and feed additives. Additionally, chemical alternatives, vaccines, bacteriophages, quorum quenching, and antibiofilm approaches are explored. The future of aquatic animal health is covered through the lens of novel technologies, including genomic and metabolomic approaches, nanotechnology, and nanobubble methods. This book provides insightful knowledge for individuals from both academic and private sectors, as well as policymakers and regulators. The knowledge gathered in this book is intended to positively guide aquaculture supply chains toward a sustainable transformation of the industry.