This book constitutes the proceedings of the 38th International Conference on Application and Theory of Petri Nets and Concurrency, PETRI NETS 2017, held in Zaragoza, Spain, in June 2017. Petri Nets 2017 is co-located with the Application of Concurrency to System Design Conference, ACSD 2017. The 16 papers, 9 theory papers, 4 application papers, and 3 tool papers, with 1 short abstract and 3 extended abstracts of invited talks presented together in this volume were carefully reviewed and selected from 33 submissions. The focus of the conference is on following topics: Simulation of Colored Petri Nets, Petri Net Tools.- Model Checking,  Liveness and Opacity,  Stochastic Petri Nets,  Specific Net Classes, and  Petri Nets for Pathways.