The two volume set LNCS 13258 and 13259 constitutes the proceedings of the International Work-Conference on the Interplay Between Natural and Artificial Computation, IWINAC 2022, held in Puerto de la Cruz, Tenerife, Spain in May – June 2022. The total of 121 contributions was carefully reviewed and selected from 203 submissions. The papers are organized in two volumes, with the following topical sub-headings: Part I: Machine Learning in Neuroscience; Neuromotor and Cognitive Disorders; Affective Analysis; Health Applications,Part II: Affective Computing in Ambient Intelligence; Bioinspired Computing Approaches; Machine Learning in Computer Vision and Robot; Deep Learning; Artificial Intelligence Applications.