This book constitutes the refereed proceedings of the 24th IFIP WG 5.5 Working Conference on Virtual Enterprises, PRO-VE 2023, held in Valencia, Spain, in September 2023. The 59 papers presented in this book were carefully reviewed and selected from 119 submissions. They provide a comprehensive overview of the major challenges in sustainability of collaborative ecosystems; risks, security and resilience in networks; collaborative value creation and services; collaborative interactions and human-centered networks; skills and enabling technologies; collaborative healthcare and agile production; and AI, digital twins, and intelligent frameworks.