The biennial CONTROLO conferences are the main events promoted by The CONTROLO 2016 – 12th Portuguese Conference on Automatic Control, Guimarães, Portugal, September 14th to 16th, was organized by Algoritmi, School of Engineering, University of Minho, in partnership with INESC TEC, and promoted by the Portuguese Association for Automatic Control – APCA, national member organization of the International Federation of Automatic Control – IFAC. The seventy-five papers published in this volume cover a wide range of topics. Thirty-one of them, of a more theoretical nature, are distributed among the first five parts: Control Theory; Optimal and Predictive Control; Fuzzy, Neural and Genetic Control; Modeling and Identification; Sensing and Estimation. The papers go from cutting-edge theoretical research to innovative control applications and show expressively how Automatic Control can be used to increase the well being of people.