This book gives a detailed insight into the implementation of nanotechnology in plant biotechnology studies. The field of plant biotechnology deals with plant conservation, propagation, improvement, and sustainable utilization through advanced techniques like plant tissue culture and genetic engineering.  While different strategies of plant tissue culture techniques have been long utilized for plant propagation and improvement, there is a need for continuous upgradation and implementation of novel techniques in a plant tissue culture system. This is essential to cater to the growing demand for plant-based products in global market, dwindling natural plant populations, shrinking of land for plant growth, and a contrasting rise in dependence on plants for sustenance of life. This has led to exploring nanotechnological interventions in plant biotechnology by incorporating nanoparticles in a plant tissue culture system, thereby enhancing plant growth, development, hardening, metabolite synthesis, genetic transformation, etc., under in vitro conditions. The book covers a range of topics related to nanoparticle utilization in plant propagation and improvement systems, which will be of interest to plant biotechnologists and botanists. Those working in the field of chemistry and phytochemistry will find special sections of biosynthesis of different nanoparticles for use in plant-based research,  their use in enhancing bioactive production, and phytotoxicity. Further, molecular biologists will find topics related to genetic transformation and stress tolerance in plants very useful.