Plants, being sessile organisms, are subjected to diverse biotic and abiotic stresses, and today climate change has resulted in a significant increase in incidences of abiotic stresses, which have strong impact on agriculture and food security. This new volume helps to provide a comprehensive understanding of the factors affecting plant responses to these stresses, taking a metabolomics approach in plants, which involves a comprehensive and systematic analysis of the complete set of metabolites present within plant tissues. The volume covers various approaches to understand the mechanisms of stresses and identification of traits, which are crucial to improve stress adaptation and tolerance in plants. The book discusses diverse aspects of abiotic stresses, particularly as metabolomics has emerged as an important research perspective. The chapters explore the application of metabolomics in comprehending plant responses to metalloid stress, heavy metal stress, plant-insect interaction, plant-pathogen interaction, high- and low-temperature stress, and regulation of reactive oxygen species (ROS) production. Also addressed are metabolomics-assisted breeding for the development of stress-tolerant crops, plant metabolome databases, and the use of mass spectrometry in studying plant tolerance to stress. With chapters written by experts in the field, Exploring Plant Stress Tolerance: A Metabolomics Approach will be an informative resource for plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study as well as for faculty and students in plant sciences.