This book constitutes the referred proceedings of the 9th Iberoamerican Workshop on Human-Computer Interaction, HCI-COLLAB 2023, held in Buenos Aires, Argentina, during September 13–15, 2023. The 23 full papers presented in this book were carefully reviewed and selected from 84 submissions. They cover a variety of topics related to HCI such as: Emotional Interfaces, Usability, Video Games and Gamification, Computational Thinking, Internet of Things (IoT), Software Engineering, ICT in Education, Augmented and Mixed Virtual Reality for Education, Emotional Interfaces, Adaptive Instructional Systems, Accessibility, Artificial Intelligence in HCI, Industry 4. 0 and HCI, Infotainment Systems, Intelligent Systems, Collaborative Work and Learning, Cognition, and Interaction, among others.