The book Laboratory Manual of Biochemistry: Methods and Techniques has been created from the 17 years of teaching and research experience of the authors. Biochemistry is a relatively new field, but the variety and significance of its research and rapid development have elevated it to a prominent position within the scientific hierarchy. As biochemistry operates at a molecular level, which is finer than what can be seen with modern optical or phase-contrast microscopes, it also encompasses other disciplines. This has reinforced the idea of an integrated approach and has been instrumental in solving biological mysteries. Biochemical techniques are employed in all branches of the biological sciences and biotechnology. Laboratory experiments are conducted both as a practical exercise and for research purposes. This first edition is organized into 11 units, each of which includes the principle, requirements, procedure, calculations, and observations for a particular experiment. At the end of each unit, there are references for additional reading. The notes section also contains important precautions, warnings, and tips. Furthermore, there are 12 appendices that provide detailed information on fundamental chemistry, buffer preparation, and other essential aspects of conducting experiments. The methods outlined in the book are suitable for conducting practical classes at the undergraduate and postgraduate levels in biochemistry, biotechnology, microbiology, agricultural sciences, environmental science, botany, zoology, nutrition, pharmaceutical science, and other subjects related to biology. This book will be a valuable resource for researchers since it covers procedures ranging from traditional basic biochemistry to cutting-edge PCR techniques.