Our knowledge of the molecular gas content in galaxies has advanced rapidly in the past decade with systematic surveys from ground-based radio facilities, coupled with advances in observations and modeling of the thermal dust emission associated with the gas. This Symposium Proceedings provides a timely overview of the latest observations of molecular gas and dust in the Milky Way and in other galaxies. It also covers related topics including the initial conditions for star formation, observational tracers of star formation and interstellar conditions, and simulations of the turbulent, multiphase interstellar medium. Featuring ten review articles by leaders in the field, and including early results and prospects for the ALMA observatory, this volume will prove especially useful for graduate students or scientists who are pursuing or planning research in this area.