The convergence of nanotechnology and nucleic acid delivery systems represents a paradigm shift in modern medicine. Traditional drug delivery mechanisms face significant challenges, such as poor bioavailability, limited targeting capabilities, and adverse side effects. Nanotechnology offers innovative solutions to these obstacles, enabling the development of effective delivery vehicles. This book explores the intricate interplay between nanotechnology and genetic medicine, offering insights into the latest advancements, methodologies, and applications. The book covers a broad spectrum of topics, from the fundamental principles of nanotechnology and nucleic acid chemistry to the design, synthesis, and characterization of various nanocarriers. Special attention is given to the practical aspects of developing nanotechnology-based delivery systems, including scalability, regulatory considerations, and clinical translation. The volume discusses the challenges in nucleic acid delivery, the role of polymers in nucleic acid delivery, the regulatory aspects related to nanomedicine and gene therapy applications, the role of artificial intelligence in optimizing nanocarriers for nucleic acid delivery, the application of AI and nanotechnology for personalized cancer care by nucleic acid delivery, as well as case studies and success stories of AI-driven approaches for nucleic acid delivery