This volume serves as an indispensable guide for researchers, students, and professionals who wish to explore the intricate world of pollen biology and its modern methodologies. Chapters detail collecting, counting, storing pollen, artificial intelligence, pollen classification, flow cytometric analysis, pollen characteristics, multispectral imaging, pollen tube growth in stiff media and measuring self-incompatibility in plants, providing solutions and methodologies for these obstacles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Comprehensive and cutting-edge, Pollen and Pollen Tube Analysis: Methods and Protocols represents a collaborative effort to advance the understanding of pollen biology using modern techniques and applications. This volume will serve as a valuable resource for those entering the fascinating field of pollen research.