This book addresses a critical gap in the literature, serving as a foundational resource for a rapidly evolving field that lacks a comprehensive reference point. It offers an in-depth exploration of research methods in exercise psychophysiology, beginning with a historical perspective before delving into key psychophysiological techniques and advanced methodologies for processing and interpreting biological data collected during exercise. Beyond methodology, the book examines core topics such as exercise tolerance, motivation, emotion regulation, attentional control, and cognition—unpacking the intricate psychophysiological mechanisms driving these phenomena. Designed to equip researchers and practitioners alike, it provides the necessary tools to design rigorous experiments and effectively apply complex psychophysiological methods in movement-related contexts. This resource is essential for anyone seeking a deeper understanding of how mind and body interact in the realm of exercise.