This volume looks at a collection of stem cell and regenerative techniques used by both novice and expert scientists. Chapters cover topics such as tissue repaired by expansion and reprogramming; induced pluripotent stem cells driven in neuronal or vascular differentiation; using mesenchymal stem cells to derive skeletal muscle, osteoblasts, and spermatogonial cells; and the technique of monitoring the development of sub-organ microenvironments in the developing pancreas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Stem Cells and Tissue Repair: Methods and Protocols, Second Edition is a valuable resource that provides readers with the latest descriptions and references for exploring this vast field in regenerative medicine.