The rapid evolution of biotechnology has ushered in an era in which the genome is no longer viewed as a static blueprint, but as a dynamic and editable system. The Genome Editing Frontier: Emerging Methods and Applications offers a comprehensive and forward-looking exploration of this transformation, tracing the field’s progression from foundational molecular tools to today’s high-precision, AI-integrated genome engineering platforms. The book presents an integrated perspective on how genome editing is reshaping medicine, agriculture, environmental science, and biotechnology. By bridging historical context with emerging innovations, it uniquely connects conceptual foundations, technological advances, and real-world applications within a single cohesive framework. Spanning the evolution from pre-CRISPR systems to next-generation digital and single-cell approaches, the volume highlights both the technical sophistication and the societal responsibilities of modern genome editing. Its interdisciplinary structure distinguishes it from conventional texts, combining functional genomics, computational biology, precision breeding, and ethical governance into a unified narrative of innovation. Key Features and New to This EditionComprehensive coverage from ZFNs and TALENs to advanced CRISPR systems, multiplex editing, and epigenome engineering. Dedicated sections on AI-driven genome design, predictive repair modeling, and computational optimization of editing efficiency. Expanded coverage of single-cell genome editing and high-resolution functional genomics. New chapters on AI-enabled CRISPR immunotherapies and deep learning applications in genome engineering. Integrated perspectives on ethical, regulatory, and biosafety frameworks guiding responsible innovation. Bringing together theory, methodology, and application, this volume serves as both a foundational reference and a forward-looking guide. It is ideally suited for graduate students, academic researchers, biotechnology professionals, and policy-engaged scientists seeking a rigorous and accessible roadmap to the future of genome editing.