This book focuses on transgenerational toxicology using Caenorhabditis elegans as a model organism. C. elegans provides a powerful system for assessing toxic effects across generations because of its high sensitivity to environmental exposures and short life cycle. This book systematically presents current knowledge on the mechanisms underlying transgenerational toxicity, including toxicant translocation, biological barriers, intergenerational signaling, metabolic regulation, GPCR and nuclear hormone receptor pathways, and epigenetic control. It integrating protective responses and molecular regulatory networks, and offers readers a comprehensive framework for understanding and investigating transgenerational toxic effects in environmental and molecular toxicology research. This book serves as a valuable reference for researchers and graduate students in related areas of research.