This manual comprises a range of techniques for research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering. Both applied and more fundamental uses of cell, tissue and organ culture are covered, and the ring-binder design allows the manual to be updated regularly with supplementary chapters. Each chapter, discussing a single technique or protocol, is written by an authority in the field and provides background information, references to the relevant literature and a guide to troubleshooting in addition to a thorough methodology. This Sixth Supplement to the Plant Tissue Culture Manual expands the existing sections B: Tissue Culture & Transformation of Crop Species, C: Propagation & Conservation of Germplasm, F: Mutant Selection, and H: Tissue Culture Techniques for Fundamental Studies.