?This book comprises selected papers of the 26th International Conference on Difference Equations and Applications, ICDEA 2021, held virtually at the University of Sarajevo, Bosnia and Herzegovina, in July 2021. The book includes the latest and significant research and achievements in difference equations, discrete dynamical systems, and their applications in various scientific disciplines.The book is interesting for Ph.D. students and researchers who want to keep up to date with the latest research, developments, and achievements in difference equations, discrete dynamical systems, and their applications, the real-world problems.