Methods for characterizing proteins are central to the early stages of drug development and are highly relevant to careers in the pharmaceutical and biotechnology industries. These skills are best learned through hands-on practice in academic laboratory settings. Analytical Biochemistry: A Practical Handbook for Undergraduates presents a laboratory course specifically designed to teach protein analytical techniques to postsecondary students in biochemistry and related fields. Readers are guided through a semester-long research project that mirrors how scientific work is conducted in both academic and industry environments. Instead of focusing on isolated weekly exercises, students work toward a single overarching goal and learn how individual approaches are integrated to solve a meaningful problem related to drug development. Along the way, readers gain practical experience applying the scientific method, designing experiments, interpreting data, and communicating scientific results, while also learning the theoretical principles underlying modern analytical procedures. Fundamental biochemical concepts—such as how protein structure affects function and how enzymes work—are reinforced throughout the project, helping students connect foundational concepts to real-world applications. Step-by-step instructions support student learning, and a companion instructor guide enables easy implementation by faculty. Ultimately, the book offers an authentic and engaging introduction to scientific discovery in protein biochemistry.