This book provides a comprehensive overview of biotechnological advances and applications in microbial proteases, offering readers a detailed understanding of their role in modern science and industry. By exploring the latest research and innovations, this volume sheds light on how microbial proteases are revolutionizing fields such as food processing, waste management, and biorefinery applications. The chapters cover a wide range of topics, including the sources and classification of microbial proteases, emerging trends in their applications, and advanced fermentation technologies for their production. Readers will discover the potential of genetic engineering and CRISPR-Cas9 techniques to enhance protease properties, as well as the use of AI and machine learning for optimization. The book also addresses the multifaceted applications of microbial proteases in dermatology, cosmetics, and the meat and poultry industries, highlighting their versatility and impact. Ideal for researchers, academics, and professionals in biotechnology, microbiology, and related fields, this book serves as an essential resource for anyone interested in the future of microbial protease technology. It is particularly relevant for those interested in the practical applications of microbial proteases and their potential to drive innovation and sustainability across various industries. This collection invites readers to consider critical questions about sustainable production, scalability, and safety considerations, making it a must-read for anyone involved in biotechnology and microbiology. It serves as a valuable resource for academics and postgraduate students engaged in microbial protease technology for food, feed, leather, waste management, and biotransformation, as well as for microbiologists, biotechnologists, environmental engineers, bioprocess engineers, analysts, biochemical engineers, and industrial entrepreneurs.