This book presents selected papers from the II International Conference "Ecosystems without Borders 2021", held at Kaliningrad State Technical University in Kaliningrad, Russia on October 5–7, 2021. Materials of the book "Ecosystems without Borders - Opportunities and Challenges" include theoretical and spatial aspects of ecosystems, features of the formation of a creative class in these conditions, as well as studies reflecting transformation of ecosystems, both in terms of innovations and in science, technology and business. This book will be of interest to a wide range of readers, such as practicing economists, students, graduate students and researchers, as well as government employees and company managers.