This book constitutes the referred proceedings of the 25th European Conference on Evolutionary Computation in Combinatorial Optimization, EvoCOP 2025, held as part of EvoStar 2025, in Trieste, Italy, during April 23–25, 2025.

The 16 full papers presented in this book were carefully reviewed and selected from 43 submissions. These papers cover a variety of topics, ranging from benchmark creation, over genetic programming, heuristics for real-world and NP-hard problems, as well as the foundations of evolutionary computation algorithms and other search heuristics, to both mixed-binary and multi-objective optimization.