This book is intended to serve as a comprehensive guide to navigate this rapidly evolving landscape of extracellular vesicles (EVs). It provides a thorough understanding of EVs, ranging from their basic biology to their cutting-edge uses in diagnostics and therapeutics. Its primary objective is to systematically bridge the foundational principles of EV biology with their practical and transformative clinical applications. The book is structured to serve a diverse readership—from students and researchers embarking on EV studies to clinicians and industry professionals seeking to harness their potential. The text contains 14 chapters, all of which have been authored by leading international experts that provide authoritative insights into their respective specialties. Early chapters provide the critical methodological backbone necessary for rigorous scientific inquiry. Later chapters address the translational momentum in the field, as well as detail how EVs are revolutionizing approaches to cancer, neurodegenerative, cardiovascular, and other diseases. Finally, the book concludes by discussing the ethical issues in EV research and application, ensuring progress is matched with responsibility. By consolidating current knowledge and highlighting future trajectories, Extracellular Vesicles: From the Laboratory to the Clinic aims to accelerate the responsible translation of EV science into tangible clinical solutions that improve patient outcomes worldwide.