With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.