This book constitutes the proceedings of the 16th International Symposium on Functional and Logic Programming, FLOPS 2022, held in Kyoto, Japan, in May 2022. The 12 papers presented in this volume were carefully reviewed and selected from 30 submissions. Additionally, the volume includes two system descriptions and a declarative pearl paper. The papers cover all aspects of the design, semantics, theory, applications, implementations, and teaching of declarative programming focusing on topics such as functional programming, logic programming, declarative programming, constraint programming, formal method, model checking, program transformation, program refinement, and type theory.