This book presents a comprehensive collection of methods, resources, and studies that use large language models (LLMs) in the field of protein bioinformatics. Reflecting the swift pace of LLM development today, the volume delves into numerous LLM-based tools to investigate proteins science, from protein language models to the prediction of protein-ligand binding sites. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice to ensure success in future research.  Authoritative and practical, Large Language Models (LLMs) in Protein Bioinformatics serves as an ideal guide for scientists seeking to tap into the potential of artificial intelligence in this vital area of biological study.