This book constitutes the proceedings of the 14th International Conference on Latent Variable Analysis and Signal Separation, LVA/ICA 2018, held in  Guildford, UK, in July 2018.The 52 full papers  were carefully reviewed and selected from 62 initial submissions. As research topics the papers encompass a wide range of general mixtures of latent variables models but also theories and tools drawn from a great variety of disciplines such as structured tensor decompositions and applications; matrix and tensor factorizations; ICA methods; nonlinear mixtures; audio data and methods; signal separation evaluation campaign; deep learning and data-driven methods; advances in phase retrieval and applications; sparsity-related methods; and biomedical data and methods.