This book provides an overview of biosurfactants as biobased compounds, and highlights novel applications in several industries, including biotech, food processing, cosmetic, oil recovery/petroleum, pharmaceutical, detergent and textile. In the past few decades, biosurfactants have emerged as promising alternative surface-active agents to chemical surfactants due to their high biodegradability, low toxicity, multi-functionality under extreme pH and temperature conditions, long-term physicochemical stability, and their production from renewable sources. This book presents the fundamental aspects (classification, structure, genetics, and properties) of biosurfactants and how their features contribute to the global bioeconomy. Chapters from expert contributors discuss the latest screening, purification and characterization methods of new biosurfactants and biosurfactant-producing fungi and bacteria, including extremophiles. Particular attention is given to the role of biosurfactants in the formulation of sustainable agrochemicals, and their application as eco-friendly mosquitocidal agents, and biopesticides. Readers will discover a perspective on the antiviral activity of biosurfactants, in which the latest in vitro and in silico studies are discussed. Readers will also find more about the foremost therapeutic attributes of biosurfactant-mediated nanoparticles as next-generation drug delivery systems. Given its breadth, this book appeals to a wide readership, from students and academic researchers to scientists and professionals from industry, interested in the study and application of microbial surfactants.