This volume provides basic and state-of-the-art methods for fundamental seed research and practical application. Chapters detail recent seed dormancy studies, basic methods for germination and longevity, seed imaging, sugar, chromatin analyses, QTL analysis, and GWAS protocols for diploid and complex hexaploid species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Seed Dormancy: Methods and Protocols aims to ensure successful results in the further study of this vital field.