This volume covers a variety of the latest scientific methods utilized in tick-borne virus (TBV) research. The chapters in this book explore detailed protocols on how to conduct in-depth research on TBVs; enhance understanding of the virus’ biology; transmission mechanisms; and the creation of effective countermeasures. Following the established Methods in Molecular Biology series format, each chapter introduces the topic, lists necessary materials and reagents, offers step-by-step reproducible laboratory protocols, and includes troubleshooting tips to help avoid common issues. Cutting-edge and thorough, Tick-Borne Viruses: Methods and Protocols is a comprehensive and innovative resource for researchers, clinicians, and public health experts interested in exploring the complexities of TBVs.