1 Introduction.- 2 Preservation of Tissue for Immunocytochemical Studies.- 3 Specimen Preparation for Immunocytochemistry.- 4 Antigen Retrieval.- 5 Immunolabeling.- 6 In Situ Hybridization: General Principles and Application of Digoxigenin-Labeled cRNA for the Detection of mRNAs.- 7 Fluorescence In Situ Hybridization.- 8 In Situ Amplification and Detection of Nucleic Acids.- 9 Image Analysis and Statistics: A Quantitative Approach to Immunocytochemistry and Related Techniques.- 10 Safety in the Laboratory.- 11 Applications of Affinity Labeling in Biomedical Sciences.