This volume LNCS 14361 and 14362 constitutes the refereed proceedings of the, 16th International Symposium, ISVC 2023, in October 2023, held at Lake Tahoe, NV, USA. The 42 full papers and 13 poster papers were carefully reviewed and selected from 120 submissions. A total of 25 papers were also accepted for oral  presentation in special tracks from 34 submissions. The following topical sections followed as:Part 1: ST: Biomedical Image Analysis Techniques for Cancer Detection, Diagnosis and Management; Visualization; Video Analysis and Event Recognition; ST: Innovations in Computer Vision & Machine Learning for Critical & Civil Infrastructures; ST: Generalization in Visual Machine Learning; Computer Graphics; Medical Image Analysis; Biometrics; Autonomous Anomaly Detection in Images; ST: Artificial Intelligence in Aerial and Orbital Imagery; ST: Data Gathering, Curation, and Generation for Computer Vision and Robotics in Precision Agriculture. Part 2: Virtual Reality; Segmentation; Applications; Object Detection and Recognition; Deep Learning; Poster.