Bioanalytical chemistry plays a central role in many fields, from healthcare to food and environmental control. This book aims to provide graduate students and young researchers with the interdisciplinary knowledge necessary to understand the principles and application of contemporary bioanalytical methodologies, especially those which focus on methods based on molecular recognition and nanobiosensing. This second edition has been expanded to include the most recent advances in molecular biosensing, including those developed to answer the urgent need for rapid and reliable analytical tools for fighting the COVID-19 pandemic. Introduces general concepts of molecular recognition in bioanalytical chemistry. Gives an overview of the core techniques and examines their strengths and weaknesses.