This updated volume explores the technically challenging study of brain tumor stem cells (BTSCs) with their unique capacity to self-renew, proliferate, and initiate tumor formation. The book collects up-to-date methodologies to isolate and propagate BTSCs and to study them using various cutting-edge techniques, in service of bringing us closer to translating the discoveries made from these cells into new therapeutic options for brain cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Brain Tumor Stem Cells: Methods and Protocols, Second Edition provides a comprehensive understanding of the skills and techniques needed to unlock data from this most informative subset of cells.