This second edition details updated methods and protocols for detection and quantification of bacteria, viruses and protozoan in different food matrices (fresh or processed) and freshwater. Chapters introduce the topic, materials, methods, a step-by-step description, figures, graphs or tables. Tips to avoid mistakes that compromise the analytical technique performance and reduce the working time, and supplies. At the end of each chapter, the key references are listed. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols. Authoritative and cutting-edge, Detection and Quantification of Bacteria, Yeast, Viruses, and Protozoan in Foods and Freshwater, Second Edition aims to provide basic and comprehensive information about protocols and methods for detection, quantification and characterization of microorganisms in foods, without disregarding the limitations or details in each specific procedure.