This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study Golgi formation, maintenance, and functions under physiological and pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Golgi: Methods and Protocols is a valuable tool for researchers in the field who wish to explore new areas of Golgi biology and for new investigators interested in exploring Golgi structure and function.