This book constitutes the thoroughly refereed proceedings of the 6th International Workshop on Hybrid Systems Biology, HSB 2019, held in Prague, Czech Republic, in April 2019.

The 8 full papers presented in this book together with 1 short paper and 3 invited papers were carefully reviewed and selected from 13 submissions. They cover topics such as: modeling and analysis of metabolic, signaling, and genetic regulatory networks in living cells; models of tissues, organs, physiological models; models and methods coping with incomplete, uncertain and heterogeneous information including learning for biological systems, parametric synthesis and inference; stochastic and hybrid models in biology; hierarchical systems for multi-scale, multi-domain analysis; abstraction, approximation, discretization, and model reduction techniques; modeling, analysis and design for synthetic biology, cyber-biological systems and biomedical studies (e.g. therapies, teleoperation)

game-theoretical frameworks and population models in biology (e.g. mixed-effects and Bayesian modeling)

biological applications of quantitative and formal analysis techniques (e.g. reachability computation, model checking, abstract interpretation, bifurcation theory, stability and sensitivity analysis)

efficient techniques for combined and heterogeneous (stochastic/deterministic, spatial/non-spatial) simulations for biological models; modeling languages and logics for biological systems with related analysis and simulation tools; and control architectures of biological systems including biology-in-the-loop systems and bio-robotics.