This book provides a comprehensive overview of the application of microfluidic technology in the analysis and manipulation of mammalian cells. It introduces the fundamental principles of microfluidics and explores their use in measuring and controlling the biochemical and biophysical properties of cells for disease diagnosis and therapeutic development. Microfluidic Technologies for Cell Analysis and Beyond centers on the comprehensive analysis of cells using microfluidic systems. It discusses microfluidics and their applications in the biochemical and biophysical analysis of both single cells and cell populations. Accessible to readers without a specialized background, Part I establishes foundations through cell analysis history and microfluidic fundamentals. Part II explores biochemical analysis advancements from single-cell proteomics to secretome profiling. Part III examines cellular biophysical properties including stiffness and migration, while Part IV covers manipulation techniques for cell movement and intracellular delivery. These chapters demonstrate how microfluidic technologies advance biology and translational medicine through adaptability and precision. This comprehensive guide serves as a key reference for researchers, students, and professionals at the intersection of biology, engineering, and medicine.