This book constitutes the proceedings of the 16th International Conference on Research Challenges in Information Sciences, RCIS 2022, which took place in Barcelona, Spain, during May 17–20, 2022. It focused on the special theme "Ethics and Trustworthiness in Information Science". The scope of RCIS is summarized by the thematic areas of information systems and their engineering; user-oriented approaches; data and information management; business process management; domain-specific information systems engineering; data science; information infrastructures, and reflective research and practice. The 35 full papers presented in this volume were carefully reviewed and selected from a total 100 submissions. The 18 Forum papers are based on 11 Forum submissions, from which 5 were selected, and the remaining 13 were transferred from the regular submissions. The 6 Doctoral Consortium papers were selected from 10 submissions to the consortium. The contributions were organized in topical sections named: Data Science and Data Management; Information Search and Analysis; Business Process Management; Business Process Mining; Digital Transformation and Smart Life; Conceptual Modelling and Ontologies; Requirements Engineering; Model-Driven Engineering; Machine Learning Applications. In addition, two-page summaries of the tutorials can be found in the back matter.