The two-volume set LNCS 14634 and 14635 constitutes the refereed proceedings of the 27th European Conference on Applications of Evolutionary Computation, EvoApplications 2024, held as part of EvoStar 2024, in Aberystwyth, UK, April 3–5, 2024, and co-located with the EvoStar events, EvoCOP, EvoMUSART, and EuroGP. The 51 full papers presented in these proceedings were carefully reviewed and selected from 77 submissions. The papers have been organized in the following topical sections: applications of evolutionary computation; analysis of evolutionary computation methods: theory, empirics, and real-world applications; computational intelligence for sustainability; evolutionary computation in edge, fog, and cloud computing; evolutionary computation in image analysis, signal processing and pattern recognition; evolutionary machine learning; machine learning and AI in digital healthcare and personalized medicine; problem landscape analysis for efficient optimization; softcomputing applied to games; and surrogate-assisted evolutionary optimisation.